Table 2.
Specificity evaluation of rhApobec3 primers and probes.
| Primer-probe set | Template cross-reactivity (input: 5 × 108 copies) | ||||||
|---|---|---|---|---|---|---|---|
| rhA3A | rhA3B* | rhA3C | rhA3DE* | rhA3F | rhA3G | rhA3H | |
| rhA3A | NT | — | NT | 9.9×101 | 2.7×103 | — | |
| rhA3B | — | — | 2.0×103 | — | — | — | |
| rhA3C | — | NT | NT | — | — | 1.5×104 | |
| rhA3D | — | 1.5×102 | — | — | — | — | |
| rhA3F | 6.5×103 | NT | — | NT | 3.4×101 | — | |
| rhA3G | — | NT | — | NT | 5.1×104 | — | |
| rhA3H | — | NT | 1.7×103 | NT | — | — | |
*
rhA3B (340nt) and rhA3DE (370nt) templates were synthetic oligos based on predicted sequences. —, no cross-reactivity observed based on endpoint-PCR and gel electrophoresis; NT: not tested.