Skip to main content
. Author manuscript; available in PMC: 2014 Jul 21.
Published in final edited form as: Virology. 2010 Aug 13;406(2):312–321. doi: 10.1016/j.virol.2010.07.021

Figure 7.

Figure 7

The down-regulation of cell surface expression of ptBST-2 mutants by Vpu. To measure surface down-regulation of BST-2 mutants in the presence of HIV-1 Vpu, 293 cells were seeded on a 12-well format and transfected using Lipofectamine 2000. For each transfection, 0.25 μg of pCG-GFP, 0.5 μg of pVphu or mock DNA plasmid, and 0.06 μg of each BST-2 noted above were used. At 24 hours, the cells were reacted with a mouse anti-HM1.24 antibody, then with an APC-conjugated goat anti-mouse antibody, followed by fixation. The cells were analyzed by two-color flow cytometry. For each BST-2 mutant, the APC mean fluorescent intensity (MFI) of high GFP expressing cells in the presence of Vphu was normalized to the MFI of cells without Vphu. The data are represented as percent BST-2 remaining on the surface of transfected cells in the presence of Vphu. All conditions were run at least twice and the standard deviation calculated.