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. Author manuscript; available in PMC: 2014 Jul 21.
Published in final edited form as: Virology. 2010 Sep 28;408(1):89–102. doi: 10.1016/j.virol.2010.08.031

Figure 1.

Figure 1

Vpu partitions into detergent resistant membrane (DRM) fractions. 293 cells were transfected with a plasmid expressing Vphu protein. At 48 hours, cells were lysed in ice cold DRM buffer containing 1% Triton X-100, and raft proteins separated from non-raft proteins on discontinuous sucrose gradients by ultracentrifugation as described in the Materials and Methods section. Fractions were collected from the top of the gradient, and Vpu proteins detected by Western blot analysis using a rabbit anti-Vpu serum (Upper panel) or stripped and reprobed with either an antibody directed against raft protein flotillin-1 (Middle panel) or the non-raft protein transferrin receptor (Lower panel). Fraction 1 is the top of the gradient and fraction 12 the bottom of the gradient.