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. Author manuscript; available in PMC: 2014 Jul 21.
Published in final edited form as: Virology. 2010 Sep 28;408(1):89–102. doi: 10.1016/j.virol.2010.08.031

Figure 10.

Figure 10

Raft association correlates with enhanced virus release. The same mutants described in Figure 7A were used to determine the level of virus release in the presence of human BST-2. HeLa cells were co-transfected with SHIVΔVpu and vectors expressing Vphu or the different transmembrane mutants. At 48 hours, the culture medium was collected and assayed for p27 and infectious virus released from cells. Panel A. The level of p27 released from transfected cells. Significance in the restriction of p27 release was calculated with respect to the SHIVKU-2MC4 in the presence of each respective BST-2 using a Student's t-test with p< 0.01 considered significant (★) .Panel B. The level of infectious virus released as determined by infection of TZM-bl cells and staining for the presence of β-galactosidase activity at 48 hours post-inoculation as described in the Materials and Methods section. Significance in the restriction of infectivity was determined with respect to the unmodified Vphu using a Student's t-test with p<0.01 considered significant (★).