Skip to main content
. Author manuscript; available in PMC: 2014 Jul 21.
Published in final edited form as: Virology. 2010 Sep 28;408(1):89–102. doi: 10.1016/j.virol.2010.08.031

Figure 11.

Figure 11

CD4 down-regulation by Vphu transmembrane mutants. HeLa CD4+ cells were transfected with plasmids expressing each of the unmodified Vphu or mutant Vphu proteins and one expressing EGFP. At 48 hours, live cells were immunostained for CD4. Cells were assessed for CD4 surface expression using flow cytometry. A mean fluorescent intensity (MFI) ratio of transfected (EGFP positive) to untransfected cells (EGFP negative)was calculated for each sample with the EGFP transfection control normalized to 1.0. Normalized ratios from three separate experiments were averaged and the standard error calculated.