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. Author manuscript; available in PMC: 2014 Jul 21.
Published in final edited form as: Virology. 2010 Sep 28;408(1):89–102. doi: 10.1016/j.virol.2010.08.031

Figure 5.

Figure 5

Co-patching experiments reveal that VpuSCEGFP1 partially co-localizes with NFP-GPI. Panels A, D, G, J, and M are fluorescent micrographs using a filter for YFP/EGFP . Panels B, E, H, K, aand N are fluorescent micrographs for Cy5. Panels C, F, I, L, and O are a merge of the two fluorescent micrographs to the left. Panels A-C. 293 cells were transfected with vector expressing VpuSCEGFP1. Note the punctuate fluorescence in Panel C. Panels D-F. 293 cells were transfected with vector expressing YFP-GPI and pre-fixed. Panels G-I. 293 cells were transfected with a vector expressing YFP-GPI and co-patched. Panels J-O. 293 cells were transfected with both VpuSCEGFP1 and NFP-GPI.