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Published in final edited form as: Virology. 2010 Sep 28;408(1):89–102. doi: 10.1016/j.virol.2010.08.031

VpuEGFPW23A and VpuEGFP are localized to similar compartments. 293 cells were transfected with a vectors expressing either VpuEGFP or VpuEGFPW23A and either ER-DsRed2, Golgi-DsRed2 or Membrane-DsRed2 as described in the Materials and Methods section. At 48 hours, cells were processed for confocal microscopy. Figure 9A-I. 293 cells transfected with VpuEGFP. Panels A, D, and G are fluorescent micrographs showing expression of VpuEGFP. Panels B, E, H are fluorescent micrographs showing expression of DsRed2 proteins. Panels C, F, and I are a merge of the two panels to the left. Panels A-C. 293 cells transfected with VpuEGFP and ER-DsRed2. Panels D-F. 293 cells transfected with VpuEGFP and Golgi-DsRed2. Panels G-I. 293 cells transfected with VpuEGFP and Mem-DsRed2. Figures 9J-R. 293 cells transfected with VpuEGFPW23A. Panels J, M, and P are fluorescent micrographs showing expression of VpuEGFPW23A. Panels K, N, and Q are fluorescent micrographs showing expression of DsRed2 proteins. Panels L, O, and R are a merge of the two panels to the left. Panels J-L. 293 cells transfected with VpuEGFPW23A and ER-DsRed2. Panels N-O. 293 cells transfected with VpuEGFPW23A and Golgi-DsRed2. Panels P-R. 293 cells transfected with VpuEGFPW23A and Mem-DsRed2.

VpuEGFPW23A and VpuEGFP are localized to similar compartments. 293 cells were transfected with a vectors expressing either VpuEGFP or VpuEGFPW23A and either ER-DsRed2, Golgi-DsRed2 or Membrane-DsRed2 as described in the Materials and Methods section. At 48 hours, cells were processed for confocal microscopy. Figure 9A-I. 293 cells transfected with VpuEGFP. Panels A, D, and G are fluorescent micrographs showing expression of VpuEGFP. Panels B, E, H are fluorescent micrographs showing expression of DsRed2 proteins. Panels C, F, and I are a merge of the two panels to the left. Panels A-C. 293 cells transfected with VpuEGFP and ER-DsRed2. Panels D-F. 293 cells transfected with VpuEGFP and Golgi-DsRed2. Panels G-I. 293 cells transfected with VpuEGFP and Mem-DsRed2. Figures 9J-R. 293 cells transfected with VpuEGFPW23A. Panels J, M, and P are fluorescent micrographs showing expression of VpuEGFPW23A. Panels K, N, and Q are fluorescent micrographs showing expression of DsRed2 proteins. Panels L, O, and R are a merge of the two panels to the left. Panels J-L. 293 cells transfected with VpuEGFPW23A and ER-DsRed2. Panels N-O. 293 cells transfected with VpuEGFPW23A and Golgi-DsRed2. Panels P-R. 293 cells transfected with VpuEGFPW23A and Mem-DsRed2.