Table 1.
Infectivity of SIV Vif N-Terminal Mutants
| Vif | ΔVif | K5A | R6A | R14A | R18A | R21A | H23A | K27A | K30A | K32A | K34A | K38A | K46A | |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| RhA3A | 100a | 7* | 86 | 44 | 90 | 47 | 44 | 76 | 86 | 100 | 78 | 46 | 93 | 88 |
| RhA3B | 100 | 100 | 79 | 80 | 66 | 100 | 76 | 100 | 100 | 100 | 100 | 95 | 100 | 100 |
| RhA3C | 100 | 91 | 100 | 63 | 60 | 100 | 53 | 100 | 100 | 100 | 48 | 70 | 70 | 62 |
| RhA3D | 100 | 2* | 57 | 24* | 0.7* | 36 | 18* | 48 | 100 | 66 | 75 | 35 | 92 | 93 |
| RhA3F | 100 | 7* | 99 | 37 | 21* | 100 | 14* | 11* | 19* | 23* | 13* | 16* | 100 | 84 |
| RhA3G | 100 | 4* | 100 | 61 | 0.1* | 100 | 57 | 77 | 1.0* | 102 | 80 | 62 | 100 | 100 |
| RhA3H | 100 | 22* | 65 | 100 | 1.0* | 93 | 37 | 16* | 79 | 25* | 100 | 14* | 98 | 70 |
Infectivity of virus expressing the unmodified Vif was considered 100%. Those values that were statistically significant are marked with an (*). Infectivity was determined by transfecting 293 cells with the vectors with the viral genome and one expressing one of the rhA3 proteins, collecting virus at 48 h and assaying for infectivity using the TZM.bl assay.