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. Author manuscript; available in PMC: 2014 Jul 21.
Published in final edited form as: Microb Pathog. 2003 Apr;34(4):179–186. doi: 10.1016/s0882-4010(03)00005-6

Fig. 1.

Fig. 1

Effect of pH on B. bacilliformis ialB mRNA levels. (A) The amount of ialB mRNA increased 231% in acid-treated bacteria (pH 5.0) as compared to bacteria kept at pH 7.2. Data are from a representative experiment comparing the amount of ialB mRNA in 4 μg of total RNA isolated from pH-treated B. bacilliformis samples. (B) NPA shows the amount of ialB mRNA increases at pH 5.0 (3 lanes on left) as compared to samples kept at pH 7.2 (3 lanes on right). NPA's were done in triplicate. (C) In a representative experiment comparing the amount of ialB mRNA in B. bailliformis samples kept at pH 7.2 or shifted to pH 8.0, there was a 29% decrease in ialB mRNA in pH 8.0 samples. (D) The amount of ialB mRNA decreased in base-treated samples (3 lanes on the left) as compared to samples kept at pH 7.2 (3 lanes on the right).