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. 2014 May;5(5-6):154–164. doi: 10.18632/genesandcancer.15

Table 2. The primer sequences of polymerase chain reactions.

Parameters Primer Sequence (5'→3') Product size Reference
β-actin f: 5' - GCA TGG AGT CCT GTG GCA T - 3' 326 49
r: 5' - CAT GAA GCA TTT GCG GTG G - 3'
Notch1 f: 5' - TGT GAC AGC CAG TGC AAC TC - 3' 577 50
r: 5' - TGG CAC TCT GGA AGC ACT GC - 3'
Notch2 f: 5' - AAT GTC ATG GCC GCT TCA GAG - 3' 533 51
r: 5' - TCG TGC AAG AGC CAG TTA CCC - 3'
Hes1 f: 5' - CCA GTT TGC TTT CCT CAT TCC - 3' 240 52
r: 5' - TCT TCT CTC CCA GTA TTC AAG TTC C - 3'
Wnt2 f: 5' - GCC ACA CGC TGC ACC TAA AGC - 3' 379 53
r: 5' - CAA TTA CCC TAA GGG TGG TAG C - 3'
Wnt5a f: 5' - CTA ACT TAG CTG TGT GGG ACA TG - 3' 254 54
r: 5' - AAA TGC AGA AAG CAA GCT AGC AG - 3'
β-catenin f: 5' - ACA AAC TGT TTT GAA AAT GG - 3' 298 55
r: 5' - CGA GTC ATT GCA TAC TGT CC -3'
STAT3 f: 5'- GGG TGG AGA AGG ACA TCA GCG GTA A - 3' 198 56
r: 5' - GCC GAC AAT ACT TTC CGA ATG C - 3'
PIAS3 f: 5' - ACG CTG TTG GCC CCT GGC AC - 3' 322 57
r: 5' - GGG GCT CGG CCC CAT TCT TGG - 3'