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. Author manuscript; available in PMC: 2015 Jun 15.
Published in final edited form as: Bioorg Med Chem. 2014 Apr 20;22(12):3123–3140. doi: 10.1016/j.bmc.2014.04.024

Figure 3.

Figure 3

Translocation of different PKC isoforms in living cells. CHO-K1 cells were transiently transfected with GFP-PKCα (A, B) and LNCaP cells were transiently transfected with YFP-PKCε (C, D), GFP- PKCδ (E), or GFP-RasGRP3 (F). The translocation pattern was examined by confocal microscopy as a function of time after treatment with the indicated drugs (10,000 nM) or PMA (1000 nM). Each panel represents images typical of the experiments performed (n = 3–6). Bars are 10 μm. Quantitative analysis of PKCα (B) and PKCε (D) translocation. Average ± SEM of calculated values are presented.