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. Author manuscript; available in PMC: 2015 Feb 1.
Published in final edited form as: Traffic. 2013 Dec 3;15(2):179–196. doi: 10.1111/tra.12138

Figure 3. Determination of single-fluorophore brightnesses.

Figure 3

A) Sample images of single fluorophores (arrows) in prism-based TIRFM illumination. Left, single AlexaFluor488-conjugated DC6 mAb; middle, single AlexaFluor488-conjugated 120507 mAb; right, single GFP. B) Sample single-step photobleaching traces of single AlexaFluor488-conjugated DC6 mAb (left), single AlexaFluor488-conjugated 120507 mAb (middle), and single GFP (right). C) Histogram plots of the brightness distributions of single fluorophores. Left, single AlexaFluor488-conjugated DC6 mAb; middle, single AlexaFluor488-conjugated 120507 mAb; right, single GFP.