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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Biomaterials. 2014 Jan 23;35(11):3688–3696. doi: 10.1016/j.biomaterials.2013.12.085

Fig. 2.

Fig. 2

Binding of C3 to DMPC liposomes was investigated by SDS-PAGE and Western blotting with a polyclonal anti-C3c antibody. Liposomes were incubated in lepirudin plasma in the absence (lane 4–5) or presence of 10 μm Compstatin (CMP; lane 6). After incubation in lepirudin plasma for 0 min (lane 4) or 30 min (lane 5–6), the liposomes were washed and subjected to SDS-PAGE under reducing conditions and blotted to a PVDF membrane. C3, C3b, and iC3b (lane 1–3) were used as controls. Addition of Compstatin did not attenuate the binding of C3 to liposomes.