Fig. 3.

Adsorption and cleavage of C3 on the liposome surface was further confirmed using DMPC liposomes and purified native C3 (200 μg/ml). Liposomes were incubated with native C3 in the absence (lane 4) or presence of factor H (32 μg/ml) and factor I (8 μg/ml) (lane 5) for 10 min. After washing, the liposomes were analyzed by SDS-PAGE and Western blotting with a polyclonal anti-C3c. C3, C3b, and iC3b (lane 1–3) were used as controls.