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. Author manuscript; available in PMC: 2014 Oct 1.
Published in final edited form as: Biomaterials. 2014 Jan 23;35(11):3688–3696. doi: 10.1016/j.biomaterials.2013.12.085

Fig. 4.

Fig. 4

Characterization of liposome-bound C3 was further investigated by the use of different mAbs. (A): 4SD17.3 (lane 3) against the C3a epitope (α1), 7D.398.1 (lane 4) against the 20 kDa C3c epitope (α2), 7D.9.2 (lane 5) against C3d (α4), and 7D.236.2 (lane 6) against the 40 kDa C3c epitope (α5). The binding was compared to that of a polyclonal anti-C3c (lane 2) and a C3 control (lane 1). Liposomes were incubated in lepirudin plasma for 30 min. After boiling, the samples were subjected to SDS-PAGE under reducing conditions and blotted to a PVDF membrane. Epitope locations for the anti-C3 mAbs is shown in panel (B).