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. 2014 Jun 30;111(28):10305–10310. doi: 10.1073/pnas.1402571111

Fig. 3.

Fig. 3.

CTSH overexpression decreases cytokine-induced apoptosis. INS-1 cells were stably transfected with an empty control vector (pcDNA) or a vector encoding CTSH (pCTSH #1 and pCTSH #2). Cells were untreated or exposed to IL-1β (150 pg/mL) and IFN-γ (5 ng/mL) (MIX) for 24 h. (A) Immunoblot of CTSH in INS-1 pcDNA and pCTSH cells. (B) Apoptosis determined by the presence of cytosolic histone–DNA complexes. Data are means ± SEMs (n = 4). (C) Caspase-3/7 activity. Data are means ± SEMs (n = 4). CTRL, control. *P < 0.05; **P < 0.01.