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. 2014 Jun 23;111(28):10371–10376. doi: 10.1073/pnas.1404657111

Fig. 1.

Fig. 1.

Class III transient targets of bZIP1 are uniquely associated with rapid N signaling. (A) Primary bZIP1 targets identified by either bZIP1-induced regulation or bZIP1 binding assayed in the same root protoplast samples. Intersection of these datasets revealed three distinct classes of primary targets: (class I) poised, TF-bound but not TF-regulated; (class II) stable, TF-bound and TF-regulated; and (class III) transient, TF-regulated but no detectable binding. Classes II and III are subdivided into activated or repressed, with their associated overrepresented GO terms (FDR < 0.01) listed. bZIP1 primary targets detected in protoplasts (A) were compared with bZIP1-regulated genes in planta (B) (15), and N-regulated genes in plants (1, 17, 24, 25) (C) [size of overlap is listed, and significance is indicated by asterisks (yellow, P < 0.001)]. Class III transient targets are uniquely enriched in genes related to rapid N signaling (1, 24). (C) Class IIIA target genes (NLP3 and NRT2.1) show transient bZIP1 binding at 1 and 5 min after nuclear import of bZIP1 but not at later time points (30 and 60 min) (D).