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. 2014 Jul 1;111(28):10209–10214. doi: 10.1073/pnas.1405158111

Fig. 3.

Fig. 3.

Glycolytic defects precede NAD+ depletion. (A) NAD+ measurements in mouse cortical neurons treated with 50 μM MNNG for 15 min. NAD+ was quantified at the indicated time points after MNNG treatment. NAD+ levels in cortical neurons at 360 min after DMSO or MNNG treatment were 142.66 ± 15.08, 37.95 ± 13.18, and 165.49 ± 20.14 pmol per million cells in DMSO-, MNNG-, and MNNG + DPQ-treated cultures, respectively. n = 4; *P < 0.01 vs. control (B) Glycolytic flux in the presence 10 mM glucose in mouse cortical neurons treated with MNNG ± DPQ was measured directly after MNNG washout in an XF 24 analyzer. n = 5, *P < 0.01 vs. control. (C) ATP measurements in MNNG-treated cortical neurons at the indicated time points. At 360 min after MNNG treatment, the ATP concentration was 0.0604 ± 0.0144 nM/μg protein, compared with 0.3499 ± 0.0481 in DMSO-treated and 0.3320 ± 0.0456 in MNNG + DPQ-treated cultures. n = 4; *P < 0.01 vs. control. Data represent mean ± SEM.