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. 2014 Jun 30;111(28):10167–10172. doi: 10.1073/pnas.1324301111

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Fig. 4. — Photochemical characterization of IlaC variants. (A) Kinetics of the dark recovery of IlaC derivatives from the lit (P_fr) state. Plotted are changes in absorbance at 755 nm over time following 5-min excitation with 700-nm light. Blue trace, IlaC22 k27; green trace, IlaC22 k27 Y259F. Half of the P_fr form of IlaC22 k27 decayed after 46 ± 3 s and half of the P_fr form of the IlaC22 k27 Y259F mutant decayed after 197 ± 9 s. (B) Plate assays of the IlaC k22 dark recovery mutants. (C) Light-induced spectral changes in IlaC22 derivatives. Black (P_r), before irradiation; red (Pf_r), after irradiation with 700-nm light; Δ, difference (P_fr − P_r) spectra. IlaC22 k27 (Left); IlaC22 k27 Y259F (Right).