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. 2014 Jun 30;111(28):10299–10304. doi: 10.1073/pnas.1404399111

Fig. 1.

Fig. 1.

BDNF stimulates migration of human lung adenocarcinoma cell lines. (A) Human lung adenocarcinoma cell lines were plated into transwell assays with the indicated medias. n = 3. *P < 0.01. (B) Serum-free (SFM) and lymph node fibroblast conditioned media (CM) were incubated on protein array membranes. n = 1. (C) A549 transwell migration assays with control CM (Control IgG IP), BDNF-depleted CM (BDNF IP), or BDNF-depleted CM supplemented with BDNF (BDNF IP + Rescue). n = 3. *P < 0.01. (D) Quantitative PCR (qPCR) analysis showing BDNF expression of the indicated sorted cell populations from wild-type mouse lungs. n = 3. *P < 0.01. (E) ELISA showing BDNF protein levels in wild-type mouse tissues (n = 4). *P < 0.01 indicates a significant difference between that tissue and the amount of BDNF in the stomach. All error bars represent mean ± SEM.