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. 2014 Jun 30;111(28):10299–10304. doi: 10.1073/pnas.1404399111

Fig. 2.

Fig. 2.

TrkB is required for human lung adenocarcinoma cell line migration and metastasis. (A) Transwell assays showing migration of the indicated cell lines expressing shTrkB toward conditioned media. n = 3. *P < 0.05. (B) Quantification of lung tumor burden of three to five mice per group i.v.-injected with H2030-BrM3 shGFP, shTrkB, or shTrkB-2 cells (n = 2–4). *P < 0.01. (C) Transwell assays showing migration of the indicated cell lines treated with ANA-12. n = 2–3. *P < 0.03. (D) Transwell assays showing migration of H23 cells overexpressing empty vector, wild-type TrkB (TrkB-OE), or kinase dead TrkB (TrkB-K571M). n = 2. *P < 0.02. (E) Representative pAkt and Akt immunoblots of the protein extracts from H2030-BrM3 shGFP and shTrkB cells treated with BDNF. n = 3. (F) Transwell assays showing migration of shGFP and shTrkB H2030-BrM3 cells expressing empty vector (EV) control or constitutively active Akt. n = 3. *P < 0.02. All error bars represent mean ± SEM.