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. 2014 Jun 30;111(28):10299–10304. doi: 10.1073/pnas.1404399111

Fig. 4.

Fig. 4.

HIF-1α induces TrkB expression in human lung adenocarcinoma cell lines. (A) qPCR analysis of TRKB expression of the indicated cell lines cultured in hypoxia or normoxia. n = 3–4. *P < 0.04. (B) qPCR analysis of TRKB expression of H2030-BrM3 cells expressing the indicated hairpins. n = 3. *P < 0.01. (C) Transwell assays showing migration of the indicated cell lines cultured in normoxia or hypoxia toward media with DMSO or K252A. n = 3. *P < 0.03. (D) qPCR using VEGF negative or positive control or TrkB promoter primers and DNA purified from HIF-1α ChIP in A549 cells incubated in normoxia or hypoxia. n = 3. The enrichment relative to β-actin and the input is shown. *P < 0.01. All error bars represent mean ± SEM.