Skip to main content
. 2014 Jun 30;111(28):E2858–E2865. doi: 10.1073/pnas.1409869111

Fig. 2.

Fig. 2.

Crosslinking of a TnsB BPA-containing peptide to various MBP-TnsC fusions. BPA-containing TnsB peptide B* was incubated with MBP-TnsC fusions containing various segments of TnsC and exposed to UV light as indicated. The reactions were then displayed on an SDS/PAGE gel, transferred to a membrane, and analyzed by Western blot analysis with antibiotin antibody that visualizes the peptide. (A) TnsB peptide B* crosslinks MBP-TnsC361–555. Lane 1, peptide B* incubated without TnsC with exposure to UV light; lanes 2, 3, and 4, peptide B* incubated with MBP-TnsC derivatives TnsC1–293, TnsC294–555, and TnsC361–555, as indicated in the absence of UV; lanes 5, 6, and 7, MBP-TnsC derivatives TnsC1–293, TnsC294–555, and TnsC361–555, incubated as indicated in the presence of UV; lanes 8, 9, and 10, peptide B* incubated with MBP-TnsC derivatives TnsC1–293, TnsC294–555, and TnsC361–555 as indicated in the presence of UV. (B) TnsB peptide B* crosslinks MBP-TnsC451–555. Lanes 1, 2, and 3, peptide B* incubated with MBP-TnsC361–555, TnsC401–555, and TnsC451–555, as indicated in the absence of UV; lanes 4, 5, and 6, peptide B* incubated with MBP-TnsC361–555, TnsC401–555, and TnsC451–555 as indicated in the presence of UV. (C) The TnsC mutant MBP-TnsC361–555L475A/L476A crosslinks poorly with the TnsB peptide B*. Lanes 1, 2, and 3, peptide B* incubated with MBP-TnsC361–555, TnsC361–555P468A/M470A, and TnsC361–555L475A/L476A, as indicated in the absence of UV; lanes 4, 5, and 6, peptide B* incubated with MBP-TnsC361–555, TnsC361–555P468A/M470A, and TnsC361–555L475A/L476A, as indicated in the presence of UV.