Figure 4.

Biophysical characterization of WcbI binding to CoA. (a) Differential scanning fluorimetry thermal denaturation curves for WcbI show a biphasic response. With no CoA (black line) or a high saturation (1 mM) of CoA (grey line), a monophasic response is observed. At intermediate concentrations of CoA (60 µM; dashed black line), a biphasic curve representing some bound and some unbound WcbI is observed. (b) WcbI shows dose-dependent binding to CoA, as expected for a single tight-binding molecule. All experiments were performed in triplicate and the data are representative of at least three experiments. The black line represents the fit of the data according to (1).