Table 1.
Characteristics of the 25 EST-SSR primers developed in Callerya speciosa.
| Locus (GenBank accession no.) | Primer sequences (5′–3′) | Repeat motif | Size range (bp) | BLAST top hit description [organism] | BLAST top hit accession no. | E -value |
| Ndl_001 | F: TCTGAAGCACCATCACCAAG | (AG)13AC(AG)2 | 213–236 | — | ||
| (JX046819) | R: TGAGGTACAAGGGTTCACGA | |||||
| Ndl_002 | F: TATCTGCTGCCACATCTTCG | (AT)10(GT)7 | 268–292 | Lectin-related polypeptide [Robinia pseudoacacia] | BAA36416.1 | 6e-74 |
| (JX046820) | R: AACCACCCACCTTGCATAAG | |||||
| Ndl_003 | F: ACCCTCTCCTTGCCCAGTAT | (AT)8 | 288–296 | Predicted homeobox protein knotted-1-like 2-like isoform 2 [Glycine max] | XP_003522219.1 | 1e-28 |
| (JX046821) | R: CCCAGCTGAACAAGAGCTTC | |||||
| Ndl_004 | F: TCCGACAATGTCAAGATCCA | (CCG)6 | 277–282 | Ethylene-responsive transcription factor [Medicago truncatula] | XP_003593630.1 | 2e-40 |
| (JX046822) | R: CCACGGGGTGGTTATAATTG | |||||
| Ndl_010 | F: TCTTGGAGGATGAAGGATGG | (TCT)5 | 289–298 | Predicted hypothetical protein [Glycine max] | XP_003535809.1 | 1e-92 |
| (JX046823) | R: GACTTCTAGTCCCCCGCTCT | |||||
| Ndl_011 | F: TTGTGGCAGATGGAACACTC | (GGT)6 | 262–275 | Predicted zinc finger protein ZAT10-like [Glycine max] | XP_003525928.1 | 8e-107 |
| (JX046824) | R: TGACACTGTCCCTACCGTCA | |||||
| Ndl_013 | F: AAGGGGATTAGGGTTTACGG | (AAC)6 | 161–189 | Cap-binding protein-like protein [Phaseolus vulgaris] | ABU54819.1 | 5e-133 |
| (JX046825) | R: GCTCTTCGGCTTCTTTGTTG | |||||
| Ndl_015 | F: AAGATCCAACAACTCAACTCTGG | (CT)11 | 226–245 | Predicted hypothetical protein [Glycine max] | XP_003549641.1 | 4e-20 |
| (JX046826) | R: TCTACACCCAGAAAGAGAGAAGG | |||||
| Ndl_017 | F: CGGAGCTACAAGGGTTCCTA | (CAG)10 | 248–276 | Predicted hypothetical protein [Glycine max] | XP_003535716.1 | 6e-88 |
| (JX046827) | R: TGCAGGGTTATGGTGAATGA | |||||
| Ndl_019 | F: CTGTGTGAACTTTCTTGTGTAACC | (AG)16 | 281–296 | ARG10 [Vigna radiata] | BAA25187.1 | 4e-141 |
| (JX046828) | R: GGTGACTCGTTGTGGTGTGT | |||||
| Ndl_020 | F: GACTTCTAGTCCCCCGCTCT | (AAG)5 | 291–298 | Predicted hypothetical protein [Glycine max] | XP_003535809.1 | 1e-92 |
| (JX046829) | R: TCTTGGAGGATGAAGGATGG | |||||
| Ndl_021 | F: GATCAGATGGCTCTGGAAGC | (GCG)6 | 235–241 | Predicted zinc finger protein ZAT10-like [Glycine max] | XP_003525928.1 | 8e-107 |
| (JX046830) | R: GCTTGACGGTAGGGACAGTG | |||||
| Ndl_022 | F: CACTGTCCCTACCGTCAAGC | (ACC)6 | 261–272 | Predicted zinc finger protein ZAT10-like [Glycine max] | XP_003525928.1 | 8e-107 |
| (JX046831) | R: TTGTGGCAGATGGAACACTC | |||||
| Ndl_028 | F: CTAGTGGCTCCAATGGTGGT | (GCA)6GAG(GCA)2 | 276–289 | Predicted protein TIME FOR COFFEE-like [Glycine max] | XP_003556039.1 | 6e-75 |
| (JX046832) | R: AATTGCAGGGGTCATCAAAG | |||||
| Ndl_031 | F: TTCAATCCGGAGCTACAAGG | (CAG)10 | 248–269 | Predicted hypothetical protein [Glycine max] | XP_003535716.1 | 7e-53 |
| (JX046833) | R: TGCAGGGTTATGGTGAATGA | |||||
| Ndl_032 | F: GCTGTTAATTTGCATAAGGGTAAGC | (TATT)6 | 263–286 | — | ||
| (JX046834) | R: CAAGGAGATCGCGAATCAAT | |||||
| Ndl_033 | F: GGAGCACTCAAAACCCAAAA | (AG)12 | 140–158 | Hypothetical protein [Glycine max] | NP_001241027.1 | 3e-95 |
| (JX046835) | R: TACGTGCATGCTCGAAGAAC | |||||
| Ndl_038 | F: GTCTCCACCTTCCAACTCCA | (CAG)2CAA(CAG)6 | 181–193 | Predicted hypothetical protein [Glycine max] | XP_003528481.1 | 4e-28 |
| (JX046836) | R: CACCTAATTGCTGCTGCTGA | |||||
| Ndl_042 | F: ATTCCATTCCCAATGGTACG | (CT)10 | 162–178 | 60S ribosomal protein L11, putative [Ricinus communis] | XP_002522234.1 | 1e-98 |
| (JX046837) | R: TCTTCTCCGAAGCCTGTTGT | |||||
| Ndl_043 | F: GGAGTTTTCAGGAAGGCACA | (AAC)7 | 242–266 | Predicted probable WRKY transcription factor 33 [Glycine max] | XP_003544908.1 | 3e-93 |
| (JX046838) | R: CCTTTCACTTGCTTTTGTCCA | |||||
| Ndl_047 | F: GCCTGTGCCTTTTCTCTCTG | (GA)11 | 235–247 | Putative transcription factor EREBP [Trifolium pratense] | BAE71206.1 | 3e-56 |
| (JX046839) | R: CTCGAACTGGGTTTCCTCAA | |||||
| Ndl_049 | F: ACTGACTCCACACCACACCA | (GAA)7(GAAG)5(AGG)2 | 182–196 | Predicted F-box protein PP2-A15-like [Glycine max] | XP_003521431.1 | 9e-66 |
| (JX046840) | R: TGGTACCCAGGTTCGATAGC | |||||
| Ndl_050 | F: GTGGTGGTGTTCCTGCTTCT | (TGT)10 | 252–275 | Hypothetical protein MTR_5g093390 [Medicago truncatula] | XP_003617600.1 | 1e-19 |
| (JX046841) | R: ACGGTGGGAACCCTCTTAAT | |||||
| Ndl_051 | F: TGGACCTCAACATGATGCTC | (CCA)6 | 192–212 | Hypothetical protein MTR_2g101130 [Medicago truncatula] | XP_003597685.1 | 1e-77 |
| (JX046842) | R: TTCCCTGCGGAGAAGAAGTA | |||||
| Ndl_053 | F: CTTAGGCGGTGGTTGATGTT | (CTT)10 | 220–246 | Hypothetical protein MTR_7g065150 [Medicago truncatula] | XP_003623160.1 | 2e-25 |
| (JX046843) | R: CCAGAAGAAGCAGAGGATGG |