Fig. 1. Gel A shows a methylated sample (band on p16M), Gel B shows a methylated sample with mixed forms (P16U + and p16M +). In both gels, Line 1 presents the ladder 50bp Track-It (Lifetech), Line 2 shows positive control for p16 unmethylated, Line 3 shows positive control for p16 methylated, Line 4 and 5 show negative controls for both primer pairs. Line 6 and 7 shows a cervical cancer sample (gel A) and a HSIL sample (Gel B).
