Figure 3.

(A) Structure of the activated 2′-NH₂-TNA nucleotide 2-MeImpntG. (B) Plot of the hydrolysis of 2-MeImpntG measured by ³¹P NMR with 100 mM HEPES pH 7.5, 150 mM NaCl, 100 mM HEI, 5 mM potassium phosphate reference, and 10% D₂O at 4 °C. The natural logarithm of the fraction of activated nucleotide remaining was fit to a line; the slope yielded a pseudo-first-order rate constant of 0.39 h^–1.