Figure 4.

Nonenzymatic primer extension of 2-MeImpntG on C₄ RNA, DNA, and TNA templates. (A) Schematic diagram of the primer extension reaction. A 5′-TAMRA-labeled DNA primer with a 3′-amino terminus was hybridized to each template and incubated with 5 mM 2-MeImpntG monomer at pH 7.5 and 4 °C with 150 mM NaCl and 100 mM HEI as catalyst. (B) Plot of the amount of primer remaining as a fraction of the total lane integration against time. The plot was fit to a single exponential decay to measure pseudo-first-order rate constants of 1.5, 0.97, and 0.57 h^–1 and plateau values of 8%, 9%, and 9% for the RNA (•), DNA (■), and TNA (▲) templates, respectively. (C) PAGE analysis of the products of the primer extension reactions.