Table 2. Comparison of the kDNA-PCR results with those obtained by the traditional diagnostic methods with samples from patients with suspected of cutaneous and mucosal leishmaniasis*.
| kDNA-PCR | Traditional diagnostic methods | |||||||
|---|---|---|---|---|---|---|---|---|
| In vitro culture | DI test | MST | ||||||
| P | N | Cont. | P | N | Sug. | P | N | |
| Positive | 22 (100%) | 41 (80.4%) | 36 (87.8%) | 47 (100%) | 5 (50%) | 15 (78.9%) | 48 97.9%) | 25 (86.2%) |
| Negative | 0 | 10 (19.6%) | 5 (12.2%) | 0 | 5 (50%) | 4 (21%) | 1 (2%) | 4 (13.8%) |
kDNA-PCR: Polymerase chain reaction using specific primers for Leihmania's kinetoplast DNA, DI test: Direct Investigation test, MST: Montenegro skin test. P: Positive result, N: Negative result; Sug.: Suggestive, Cont.: contamination.
*
The same sample could be analyzed by more than one diagnostic method.