Fig. 3. Gel electrophoresis of amplicons from FLA PCR conditions. A: N - negative control. Lane P (positive control) and lane 1 (cmf1) showing the specific bands (1 kb) for Acanthamoeba. Lane 2 (cmf2) showing negative result. B: Lanes 3, 4, 5 and 6 (cmf3, cmf4, cmf5 and cmf6, respectively) showing the bands (∼800 bp) for other free-living amoebae. Lane 7 (cmf7) showing negative result. M - 100 bp Ladder DNA (Fermentas®).
