Table 4.
Virus distribution in tissues of Gaoyou ducks of different ages inoculated either intramuscularly or intranasally and intraocularly on days 3 and 5 PI
| Age (days) | Inoculation route 1 | Days PI | Brain | Live | Spleen | Pancreas | Heart | Kidney | Thymus | Harderian gland | Bursa of Fabricius | Lung | Laryngotrachea | Small intestine |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 15 |
IM |
3 |
-2 (0/2)3 |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
+4 (1/2) |
+ (2/2) |
+ (2/2) |
+ (2/2) |
– (0/2) |
+ (2/2) |
+ (1/2) |
| 5 |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
+ (1/2) |
+ (2/2) |
+ (2/2) |
– (0/2) |
+ (2/2) |
– (0/2) |
||
| IN & IO |
3 |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
+ (1/2) |
+ (1/2) |
– (0/2) |
+ (1/2) |
– (0/2) |
|
| 5 |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
+ (2/2) |
– (0/2) |
+ (2/2) |
– (0/2) |
– (0/2) |
– (0/2) |
||
| 30 |
IM |
3 |
– (0/2) |
– (0/2) |
– (0/2) |
+ (1/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
+ (2/2) |
– (0/2) |
– (0/2) |
– (0/2) |
| 5 |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
||
| IN & IO |
3 |
– (0/2) |
– (0/2) |
– (0/2) |
+ (1/2) |
+ (1/2) |
+ (1/2) |
– (0/2) |
– (0/2) |
+ (1/2) |
– (0/2) |
– (0/2) |
– (0/2) |
|
| 5 |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
||
| 45 |
IM |
3 |
+ (1/2) |
– (0/2) |
+ (1/2) |
– (0/2) |
+ (1/2) |
+ (1/2) |
+ (2/2) |
+ (1/2) |
+ (1/2) |
+ (1/2) |
+ (1/2) |
– (0/2) |
| 5 |
– (0/2) |
– (0/2) |
– (0/2) |
+ (1/2) |
– (0/2) |
– (0/2) |
– (0/2) |
+ (1/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
||
| IN & IO |
3 |
– (0/2) |
– (0/2) |
+ (1/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
|
| 5 |
– (0/2) |
– (0/2) |
– (0/2) |
+ (1/2) |
– (0/2) |
– (0/2) |
– (0/2) |
+ (1/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
||
| 60 |
IM |
3 |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
+ (1/2) |
– (0/2) |
– (0/2) |
+ (1/2) |
– (0/2) |
| 5 |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
||
| IN & IO |
3 |
– (0/2) |
– (0/2) |
– (0/2) |
+ (2/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
|
| 5 |
– (0/2) |
– (0/2) |
– (0/2) |
+ (1/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
||
| 110 | IM |
3 |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
+(1/2) |
– (0/2) |
– (0/2) |
+(1/2) |
– (0/2) |
– (0/2) |
– (0/2) |
| 5 |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
||
| IN & IO | 3 |
– (0/2) |
– (0/2) |
– (0/2) |
+(1/2) |
– (0/2) |
– (0/2) |
+ (1/2) |
+ (1/2) |
– (0/2) |
– (0/2) |
– (0/2) |
– (0/2) |
|
| 5 | – (0/2) | – (0/2) | – (0/2) | – (0/2) | – (0/2) | – (0/2) | – (0/2) | – (0/2) | – (0/2) | – (0/2) | – (0/2) | – (0/2) |
1IM: inoculated intramuscularly; IN & IO: inoculated intranasally and intraocularly.
2Both samples were virus isolation negative.
3Number of positive samples/total number of samples.
4At least one sample was virus isolation positive.