A fibrocartilage defect model was formed by press fitting 3 mm punches of
6-wk-old neofibrocartilage into same-size native fibrocartilage defects and grown either
in vitro or in vivo for an additional 6 wk
(A). Control (none), LOX, C-ABC+TGF-β1, or
LOX+C-ABC+TGF-β1 pre-treatments were applied to neofibrocartilage prior to
integration assembly formation and in vitro culture in Phase 2
(B). Control (none), single LOX+C-ABC+TGF-β1, or double
LOX+C-ABC+ TGF-β1 pre-treatments were applied to neofibrocartilage prior to
integration assembly formation and in vivo culture in the backs of
athymic mice in Phase 3 (C).