Table 2. Primer sequences for bisulfite sequencing or pyrosequencing.
| Genes | Method | Sequences (5’-3’) | Annealing Tm (°C) | Product size (bp) |
|---|---|---|---|---|
|
DUSP22
(1st site) |
bisulfite sequencing |
F: GGGGAGTTTTTAGAGATTAGGTTTTT
R: AATCTCCAAATCCCCCTTTAAC |
71 |
131 |
|
DUSP22
(2nd site) |
bisulfite sequencing |
F: GTATAGAAAGTTTTGTTTTTTA
R: TATTCATCCCATTCCCCATAATA |
81 |
226 |
|
IRAK1 |
pyrosequencing |
F: TTAAATGAGGGTTGGGGTAGTAGTAA
R: ACAACAACCTTAAACCATTCAATCTC |
69 |
109 |
| CXCL6 | pyrosequencing | F: GGTTATTGGAGAGGAGGAGTATTT R: CAACAAAATCTCATCCCCTAAACTTA | 68 | 95 |