Table 1.

Statistics of RNA-seq based sequencing, assembling and functional annotation for An. sinensis

Sequencing results	Number of total raw reads	60,866,926
	Number of total clean reads	51,606,364
	Number of total clean nucleotides (nt)	4,644,572,760
	Q20 percentage of total clean reads	95.92%
	GC percentage of total clean nucleotides	51.26%
	N percentage of total clean nucleotides	0.00%
Assembling results	Number of unigenes	38,504 (5,372 into distinct clusters; 33,132 singletons)
	Total length (nt) of total unigenes	21,977,286
	Mean length (nt) of total unigenes	571
	N50 (nt) of total unigenes	711
Annotation	Unigenes with Nr database	25,456 (66% of 38,504 unigenes)
(E-value < =1e-5)	Unigenes with Nt database	20,554 (53%)
	Unigenes with Swiss-Prot database	17,651 (46%)
	Unigenes with KEGG database	16,622 (43%), 257 pathways
	Unigenes with COG database	7,204 (19%), 25 functional categories
	Unigenes with GO database	16,588 (43%), 62 subcategories grouped to 3 main categories
	Biological process	27 sub-categories
	Cellular component	17 sub-categories
	Molecular function	18 sub-categories
	Total unigenes annotated	26,650 (69% of 38,504 unigenes)