Phosphorylation is required for WspR–YFP cluster formation.
A. The localization of WspR–YFP in ΔwspF wspR–yfp or ΔwspF ΔwspA wspR–yfp mutant cells and the localization of various WspR–YFP mutant proteins in ΔwspF cells. Phase-contrast images of cells are shown on the left. The marker bar is 1 μm.
B. Colony morphologies of the strains.
C. WspR–YFP cluster formation is restored in a ΔwspF ΔwspA wspR–yfp mutant strain by providing WspA in trans.
D. The colony morphologies of the ΔwspF ΔwspA wspR–yfp strain complemented with empty vector or wspA.