Table 1.
Quantitative analysis of WspR–YFP cluster formation in wild-type and ΔwspF mutant cells grown in liquid cultures.
| Strain | Percentage of cells with WspR–YFP localized in clustersa | Total cells scored |
|---|---|---|
| wspR–yfp | 1.45 (±1.3) | 417 |
| Δ wspF wspR–yfp | 19.73 (±4.8) | 484 |
| Δ wspF ΔwspA wspR–yfp | 2 (±1.67) | 426 |
| Δ wspF wspR[D70N]–yfp | 1.5 (±0.7) | 236 |
| Δ wspF wspR[E253A]–yfp | 35.5 (±13.4) | 115 |
| ΔwspA wspR–yfp/pJN105 | 0.45 (±0.6) | 262 |
| ΔwspA wspR–yfp/pJNspA | 1.58 (±0.9) | 571 |
| ΔwspF ΔwspA wspR–yfp/pJN105 | 1.25 (±0.07) | 151 |
| ΔwspF ΔwspA wspR–yfp/pWspA | 19.4 (±14.9) | 548 |
WspR–YFP clustering was scored based on the presence of a well-defined fluorescent spot or spots in cells. This evaluation was based on the measurement of maximum and average signal intensities in single cells as described in the Experimental procedures. After calculating ratios of maximum to average signal intensities, a ratio value corresponding to presence of a well-defined spot was used as a threshold to sort the cells. At least two independent experiments were performed. Average values are given for the percentage of cells that scored above the ratio threshold value. Standard deviations are indicated in parentheses. Cells were grown in LB broth.