Table 2.
Quantitative analysis of WspR–YFP cluster formation in various strains grown on agar surfaces.
| Strain | Percentage of cells with WspR–YFP localized in clustersa | Total cells scored |
|---|---|---|
| wspR–yfp | 29.7 (±2.9) | 692 |
| Δ wspF wspR–yfp | 59.8 (±11.8) | 493 |
| Δ wspA wspR–yfp | 0.22 (±0.3) | 253 |
| Δ wspF ΔwspA wspR–yfp | 1.32 (±1.2) | 270 |
| Δ wspF wspR[E253A]–yfp | 74.95 (±1.9) | 290 |
| ΔwspA wspR–yfp/pJN105 | 0.5 (±0.7) | 299 |
| ΔwspA wspR–yfp/pWspA | 37.6 (±4.3) | 280 |
| ΔwspF ΔwspA wspR–yfp/pJN105 | 0.3 (±0.4) | 190 |
| ΔwspF ΔwspA wspR–yfp/pWspA | 58.1 (±4.6) | 414 |
WspR–YFP clustering was scored based on the presence of a well-defined fluorescent spot in cells. This evaluation was based on the measurement of maximum and average signal intensities of single cells as described in the Experimental procedures. After calculating ratios of maximum to average signal intensities, a ratio value corresponding to presence of a well-defined spot was used as a threshold to sort the cells. At least two independent experiments were performed. Average values are given for the percentage of cells that scored above the ratio threshold value. Standard deviations are indicated in parentheses. Cells were grown on LB medium solidified with 2.5% agar as described in Experimental procedures.