Table 3.

Strains.

Strain	Relative genotype	Source and/or reference
PA01	Wild-type; twitching motility+	Iglewski (Stover etal., 2000)
PAO1100	Δ wspF	Hickman etal. (2005)
PAO1101	Δ wspA	Hickman etal. (2005)
PAO1102	Δ wspE	Hickman etal. (2005)
PAO1103	Δ wspR	Hickman etal. (2005)
PAO1104	Δ wspF AwspR	Hickman etal. (2005)
PAO1251	wspR–yfp	PAO1 (this study)
PAO1252	Δ wspA wspR–yfp	PAO1101 (this study)
PAO1254	Δ wspF wspR–yfp	PAO1100 (this study)
PAO1258	Δ wspF wspR–yfp wspA-cfp	PAO1254 (this study)
PAO1260	Δ wspF AwspA wspR–yfp	PAO1254 (this study)
PAO1262	Δ wspF AwspA	PAO1100 (this study)
PAO1265	Δ wspF Ache A wspR–yfp	PAO1254 (Ferrández etal., 2002; this study)
PAO1268	Δ wspF wspR[D70N]–yfp	PAO1100 (this study)
PAO1275	wspA–yfp	PAO1 (this study)
PAO1276	Δ wspE wspA–yfp	PAO1102 (this study)
PAO1287	Δ wspF wspR[E253A]–yfp	PAO1100 (this study)
PAO1290	Δ wspF Apel Apsl wspR–yfp	PAO1543 (this study)
PAO1501	ΔwspF ΔwspA/pJN105	PAO1262 (this study)
PAO1512	ΔwspF ΔwspA wspR–yfp/pJN105	PAO1260 (this study)
PAO1514	A wspA wspR–yfp/pJ N105	PAO1252 (this study)
PAO1516	ΔwspF ΔwspA/pWspA(truncated)	PAO1262 (this study)
PAO1526	fliC∷Tn5 wspR–yfp	PAO1540 (this study)
PAO1529	Δ pilA wspR–yfp	PAO1541 (this study)
PAO1530	Δ pel Δpsl wspR–yfp	PAO1542 (this study)
PAO1534	Δ wspF ΔwspA/pWspA	PAO1262 (this study)
PAO1535	Δ wspA wspR–yfp/pWspA	PAO1252 (this study)
PAO1536	Δ wspF ΔwspA wspR–yfp/pWspA	PAO1260 (this study)
PAO1537	Δ wspF wspA–yfp	PAO1100 (this study)
PAO1539	Δ wspF ΔwspB wspA–yfp	PAO1537 (this study)
PAO1540	fliC∷Tn5	L. L. McCarter
PAO1541	Δ pilA	M. Parsek
PAO1542	Δ pel Δpsl	M. Parsek
PAO1543	Δ wspF Δpel Δpsl	PAO1542 (this study)