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. Author manuscript; available in PMC: 2014 Oct 16.
Published in final edited form as: Neuron. 2013 Sep 19;80(2):385–401. doi: 10.1016/j.neuron.2013.08.035

Figure 4. Effect of WT and mutant forms of ADAM10 expression on Aβ plaque load in AD mice.

Figure 4

(A) Aβ plaques were detected by anti-Aβ antibodies (red) in 12 month-old (first row, cortex) and 20 month-old (second and fourth rows, cortex/hippocampus) in Tg2576 single or Tg2576/ADAM10 double transgenic mice. Dense neuritic plaques were stained by Thioflavin S (green, third and fourth rows). Large and small arrows indicate neuritic and diffuse plaques, respectively. DAPI (blue) was used for nuclei counter staining. Bar= 100 µm. (B) Counts of total Aβ plaque numbers in 12 month-old mice (6–12 sections/mouse). Red: plaque size > 50 µm, blue: < 50 µm. Analyzed mouse number: Tg2576 (n=4M, 4F), Tg2576/WT (3M, 2F), Tg2576/170 (3M, 3F), Tg2576/DN (3M, 3F). *p < 0.05, **p < 0.01 (t -test). (C) Aβ plaque load in the cortex and hippocampus of 18–20 month-old Tg2576/ADAM10 mice. Analyzed mouse number: Tg2576 (n=5M, 5F), Tg2576/WT (2M, 2F), Tg2576/170 (2M, 3F), Tg2576/DN (3M, 3F). *p < 0.05, **p < 0.01, ***p < 0.001 (t-test). (D) Brain sections of Tg2576/170 (upper) and Tg2576/DN (lower) mice were immunolabeled with 3D6 Aβ antibody followed by staining with Thioflavin S. (E) Counts of Thioflavin S-stained plaque in 18–20 month old mice. ***p < 0.001 (t-test).