Table 1.

Characterization of 15 microsatellite markers developed in the genus Tilia.

Locus	Primer sequences (5′–3′)	Repeat motif	Size (bp)a	Primer conc. (mM)	Fluorescent dyeb	Multiplex sets	GenBank accession no.
Tc4	F: ATTTTAGAATGCCAACCTGCTAAG	T6(GT)12	224	0.2	HEX	B	JQ289157
	R: TATTGAAGTCCATTTCCAATTGTC
Tc5	F: TTTTCATACATTTAGAGACTTTTAGCA	(AG)12	150	0.2	FAM	D	JQ289158
	R: TGCATGATTTGTATGTTTAGGG
Tc6	F: CCATATCTTCTGCCAGTTTTCC	(AG)12	143	0.2	HEX	A	JQ289159
	R: GGACTAATTTCTTCCTTTTATTAGGC
Tc7	F: TTTACTTTTGCCAGTTGTGAGG	(GA)13	234	0.1	FAM	D	JQ289160
	R: CACCTAGAATGCCTCCTATTCG
Tc8	F: CGAAGAAACTGTCAAAACAACG	(GA)13	160	0.1	HEX	B	JQ289161
	R: AGCTGGGTTTTAGAGGATAGGG
Tc11	F: AGCTATGAAAGAACTATCAAGAGAAAG	(AG)13	146	0.1	NED	C	JQ289162
	R: CCCCAAGACATTGCAGTAGAAC
Tc31	F: TTTGCAAAGACTACTCCAAGAATC	(GA)12	205	0.2	FAM	B	JQ289164
	R: AAATCGATGGTCAAGAACTAAATC
Tc915	F: ACATCGATTGTATTTCCCTTTAAC	(CT)16	165	0.2	HEX	C	JQ289165
	R: GTTGTATTTTGCCCTTAACATTG
Tc918	F: AACGGCTAATTACTCCTAGTTTCG	(AC)9(TC)2	240	0.2	HEX	A	JQ289166
	R: TGTTCAGCTCACTACTACCTTTCAC
Tc920	F: AAATGTCTTCAGAGTGACTAGATGG	(GA)2(GT)15(AG)4	232	0.1	FAM	A	JQ289167
	R: TGCCTCATTATTCTCCTAATTCTC
Tc927	F: AGTCCTCCTGTCAAATGCTG	(AG)10	157	0.1	FAM	C	JQ289168
	R: ATCACACTCGTTTATGACATCTTG
Tc937	F: AGCCAACCAACTTTTACAATACAG	(AG)13	162	0.1	NED	A	JQ289169
	R: AGATAAAAGCACATAAATCGATGG
Tc943	F: ATTTCATCTTTCTCTAAAGCCTTG	(CA)10	150	0.2	FAM	B	JQ289170
	R: GGGAAAGCCTGTGTTAGTTTC
Tc951	F: TGTTATGACCTCACTTATAACCAAGT	(CT)12	160	0.2	NED	D	JQ289171
	R: GGGTGAGCTGACAATATAGAAGAG
Tc963	F: CTAACCCCATTCTCTTTAATTCTG	(CT)11	238	0.2	HEX	C	JQ289172
	R: GCTTTCATTTCAGTTTTCCTCTAC

^aSize of the original fragment.

^bFluorescent label on the forward primer.