A. Changes in red cell elongation index (EI) between fresh and stored cells, measured based on the length and width of the diffraction pattern, as (L1−L2)/(L1+L2), where L1 and L2 are the length and width of the diffraction pattern. B. Viscosity of red cells suspension at 30% Hct. C. Cell deformability measured using the resistance of red cells to pass by pore, normalized by the cell concentration and cell/pore volume, called β factor.38 D. Red cell membrane shear elastic modulus based on the relationship pressure and aspiration length.16 E. Cell volume measure using the cell aspiration technique. F. Red cells osmotic fragility and osmotic fragility index.