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. Author manuscript; available in PMC: 2015 May 5.
Published in final edited form as: Chembiochem. 2014 Apr 1;15(7):995–1000. doi: 10.1002/cbic.201300729

Figure 3.

Figure 3

Polypeptide backbone assignments of [80%-2H,u-13C,u-15N]-OmpA using the 3D [15N,1H]-TROSY-HNCA NMR experiment. A) 1.0 mM OmpA in Fos-10 micelles. B) 0.7 mM OmpA in DMPC nanodiscs. [ω2(13C), ω3(1H)]-strips at the indicated 15N frequencies are shown. Sequence-specific resonance assignments are indicated at the top of each strip, and red lines indicate sequential connectivities. 70(t1) × 60(t2) × 2048(t3) complex points were accumulated, with t1max(15N) = 11.9, t2max(13C) = 4.7, t3max(1H) = 71.3 ms. 64 scans per increment were acquired, resulting in a total measuring time of 69 h per experiment.