Table 5. Primer sequences of amplified genes, PCR conditions used and product size of each amplicon.

Gene	Primers 5′…….3′	Amplification conditions			Product size (bp)	References
		Temp	Time	No. of cycles
InvA	139: GTGAAATTAT CGCCACGTTC GGGCAA 141: TCATCGCACC GTCAAAGGAA CC	94 °C	5 min	1	284	22
		94 °C	30 s	35
		55 °C	45 s
		72 °C	45 s
		72 °C	10 min	1
SefA	Sef167: AGGTTCAGGC AGCGGTTACT Sef478: GGGACATTTA GCGTTTCTT	94 °C	45 s	1	312	23
		94 °C	20 s	35
		57 °C	15 s
		72 °	15 s
		72 °C	2 min	1
16S rRNA	Lwp57: AGTTTGATCC TGGCTCAG Lwp58: AGGCCCGGGA ACGTATTCAC	94 °C	2 min	1	1381	24
		94 °C	2 min	40
		55 °C	2 min
		72 °	1 min
		72 °C	7 min	1