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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Mol Carcinog. 2013 Mar 8;53(9):722–735. doi: 10.1002/mc.22024

Figure 4.

Figure 4

Effect of belinostat on the expression levels of acetylated histone 3, phospho-4EBP1, -Erk, and -p70S6k. Panel (A): Western blots were probed with antibodies directed against acetyl-H3 (acetylated histone 3) and phospho-4EBP1. Cell lysates were prepared after 24 h treatment of pancreatic cancer cells with 1 μM belinostat. Panel (B): Western blot analysis for phospho-Erk, and phospho-p70S6k. Cells were serum-starved overnight, cultured with belinostat for 4 h before EGF (15 ng/mL) was added for an additional 5 min. Panel (C): Western blot analysis for phospho-EGFR and total EGFR. Following serum starvation overnight, cells were pretreated with 1 μM belinostat for 4 h and stimulated with EGF (15 ng/mL) for 5 min.