Table 1.
Characteristics of the 10 microsatellites developed in Canavalia cathartica.
| Locus | Primer sequences (5′–3′) | Repeat motif | WellRED dyea | Ta (°C) | Size range (bp)b | GenBank accession no. |
| CANA1 | F: TTTCATTTGCTGGTGAACGC | (GT)30 | D2 | 55 | 83–111 | AB618267 |
| R: CTTCATTGACCCATACACAC | ||||||
| CANA2 | F: ATGGAGGGTTCGGTGGGGAGA | (AG)16 | D2 | 58 | 214–236 | AB618268 |
| R: CACCACCATCCTTTCCCTTTT | ||||||
| CANA3 | F: TTAGATGTTGCAAGTGCATGGG | (GT)30 | D4 | 55 | 208–260 | AB618269 |
| R: GCAGCATTTCAAACTTGGGA | ||||||
| CANA4 | F: ATTTATGACCAAATAGTCTC | (AG)35 | D3 | 55 | 252–296 | AB618270 |
| R: ACCCCTGAAAAAGTGGTGTGT | ||||||
| CANA5 | F: CAACAACAACGAAGAGGAAGA | (AC)19 | D2 | 55 | 267–293 | AB618271 |
| R: GAGGTGGACACACACTCCTT | ||||||
| CANA6 | F: TCAAATTGGTCACAACTCGC | (TGC)11 | D2 | 55 | 148–196 | AB618272 |
| R: ATGAACAGCCAGAATGCATC | ||||||
| CANA7 | F: TGTTTTTCCAGTGCCGGGAGGA | (CAG)11 | D4 | 60 | 255–294 | AB618273 |
| R: AATTTTTCTCCTCCTAACCCAGCC | ||||||
| CANA-C1 | F: ACACACACACACAGAGAGAGAG | (AC)6(AG)18 | D3 | 60 | 212–240 | AB618274 |
| R: TTGGATTCTTCTTCTTCACCTTCCG | ||||||
| CANA-C2 | F: ACACACACACACAGAGAGAGAG | (AC)6(AG)14 | D3 | 55 | 126–154 | AB618275 |
| R: CTGATAGATCCTCAGGGGAA | ||||||
| CANA-C3 | F: ACACACACACACAGAGAGAGAG | (AC)6(AG)7 | D4 | 55 | 134–140 | AB618276 |
| R: TCTGCTATCTGTTTTGCCC |
Note: Ta = annealing temperature.
a
Forward primer label.
b
Observed size range from 34 samples of C. cathartica.