Fig. 3.

Image of several patterns amplified after the first enrichment (step 5) using different concentrations of template (ligation reaction) and the primers EcoI-0 and MseI-0 (preselective modified primers; Vos et al., 1995). For each amplification template, 4 μL was loaded. The first lane is a 100-bp DNA ladder (BenchTop 100-bp DNA Ladder, Promega Corporation, Madison, Wisconsin, USA), and the last lane is Marker II (AppliChem GmbH, Darmstadt, Germany). The numbers above the white line indicate the quantity of ligation template used for the PCR enrichment, and numbers below the white line indicate the number of PCR cycles.