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. 2013 Jan 2;1(1):apps.1200179. doi: 10.3732/apps.1200179

Table 1.

Statistics of the 454 transcriptome sequencing run and metrics of the Newbler assembly software.

Size (nt) in quantiles
Sequence type Number % Nucleotides Average (nt) 0% 25% 50% 75% 100%
Reads trimmed 1521698 100 546346058 359.0 <21 <303 <395 <444 <1088
Reads assembled 381808 25.1
Reads partial 619615 40.7
Reads singleton 484576 31.8 175198711 361.6 <50 <307 < 397 < 443 <876
Reads repeat 1617 0.1
Reads outlier 20389 1.3
Reads too short 13693 0.9
Contigs 25149 12511848 498 <100 <365 <468 <601 <2394
N50 Contiga 704
a

Half of all bases are assembled in contigs of this size or longer.