Table 1.
Characteristics of 15 polymorphic and seven monomorphic ESSRs developed in Spartina alterniflora.
| ESSR locus | Primer sequences (5′–3′) | Repeat motif | Size (bp) | Ta (°C) | Location | A | PIC | GenBank accession no. | Putative function |
| SaESP01 | F: TATCCCCAGACACCCACAGT | (TC)10 | 268 | 60 | 3′ UTR | 6 | 0.70 | JU981477 | grancalcin |
| R: CATTCTCTGGGTCTGCAACA | |||||||||
| SaESP02 | F: GAAGAGACCGTTCAGGTTGG | (TGA)6 | 214 | 60 | 5′ UTR | 3 | 0.42 | JU981475 | cytochrome c |
| R: CCCGGCGACTAACTCTCAT | |||||||||
| SaESP03 | F: CGTGCCGACCAAGTAAAGTT | (CTC)6 | 256 | 60 | ORF | 4 | 0.53 | JU981468 | AIR12 precursor |
| R: ACCGACAGCGTGTTCCTC | |||||||||
| SaESP04 | F: AGCGAAGGGAAGATCTCGAC | (TC)9 | 155 | 60 | 5′ UTR | 3 | — | JW662119 | acyl-desaturase |
| R: GGAGGCCTTTTTAATAGCCG | |||||||||
| SaESP05 | F: CTTGCAGCGGCTATCCTTAC | (GCG)6 | 245 | 60 | ORF | 1 | — | JW662116 | DNA binding protein |
| R: ACGAGACCTTCGCTTTTGAA | |||||||||
| SaESP06 | F: AACCTGAAGTGCGTAAGCGT | (AT)9 | 245 | 60 | ORF | 5 | 0.46 | JU981466 | large secreted protein, putative |
| R: CTTCCCCCAACACTTCGATA | |||||||||
| SaESP07 | F: CCCAGCACCTCTGATTTGAT | (TTC)6 | 132 | 60 | 3′ UTR | 3 | 0.58 | JU981470 | bZIP transcription factor |
| R: ATCCACCTCTACCATGCGTC | |||||||||
| SaESP08 | F: TGCTAAGATTGGAGCAGGGT | (TAC)6 | 266 | 60 | 3′ UTR | 1 | — | JW662120 | serine acetyltransferase 3 |
| R: GCTTACATTACCGCCAAAGC | |||||||||
| SaESP09 | F: GACTTTACCGCGAAGAGCC | (TTC)7 | 192 | 60 | 5′ UTR | 3 | 0.43 | JU981464 | mitogen-activated protein kinase kinase kinase 2–like |
| R: AGGAAGCCCAAAACACACAC | |||||||||
| SaESP10 | F: CGAAAGGTTAAGCCAATCCA | (CT)9 | 211 | 60 | 5′ UTR | 8 | 0.82 | JU981473 | hypothetical protein |
| R: ACGAAAGTTGCGGGTACAAC | |||||||||
| SaESP11 | F: ACAAACTCGGCCTCCTCTTT | (CT)8 | 171 | 60 | 5′ UTR | 3 | — | JW662118 | CBL-interacting protein kinase 1 |
| R: ATAAGTACCCGCCCTTGTCC | |||||||||
| SaESP12 | F: GGAGCAACAAAGACAGAGCC | (CAC)6 | 215 | 60 | ORF | 1 | — | JW662115 | hypothetical protein |
| R: CGACTCGTGGTTGGTGAAG | |||||||||
| SaESP13 | F: CGATCCACTGGTACTGGGAC | (TGCC)5 | 196 | 60 | 3′ UTR | 2 | 0.37 | JU981471 | ribokinase |
| R: GGCTGCCATTATCGATTGTT | |||||||||
| SaESP14 | F: TCGTCACGTTGACTTGTGGT | (GA)8 | 249 | 60 | 3′ UTR | 4 | 0.62 | JU981469 | hypothetical protein |
| R: TGCTGCTTCCCTTTGATCTT | |||||||||
| SaESP17 | F: TGCTTCATGCGTTGATTAGC | (CA)9 | 150 | 60 | 3′ UTR | 5 | 0.58 | JU981465 | glycosyltransferase |
| R: TGAGATGAAGCCTGTGGAGA | |||||||||
| SaESP18 | F: GCCACAACAAGAGTTGGGTT | (AAT)6 | 171 | 60 | 3′ UTR | 4 | 0.45 | JU981474 | transcription factor |
| R: GCTGGTCCAAAGAAATCAGA | |||||||||
| SaESP19 | F: GCGCCATTACCACAGAGG | (CT)7 | 165 | 60 | 5′ UTR | 5 | 0.63 | JU981467 | choline/ethanolamine kinase |
| R: ATACGATCTGCCCTGTTTCG | |||||||||
| SaESP20 | F: TGTAGCTGTTAGCATTGGCG | (AG)10 | 170 | 60 | 3′ UTR | 3 | 0.54 | JU981476 | transcription factor |
| R: AGGACCAGCAGAGGACAGAG | |||||||||
| SaESP21 | F: ATACCGCAACGAAAGCAAAG | (TTC)6 | 205 | 60 | 3′ UTR | 1 | — | JW662114 | xyloglucan xyloglucosyl transferase |
| R: CTACTGCACCGACAAGTGGA | |||||||||
| SaESP22 | F: CCAGCGTCTCCTCTACAACC | (TTC)6 | 277 | 60 | 3′ UTR | 1 | — | JW662117 | hypothetical protein |
| R: CAGGAAACAAACGGACATGA | |||||||||
| SaESP23 | F: ATCCGTGCGTCTCTGTCTCT | (GTCA)5 | 255 | 60 | 3′ UTR | 2 | 0.24 | JU981478 | cyclophilin |
| R: CCACCATGATGCATAACAGC | |||||||||
| SaESP24 | F: ACCCTGCTAGATATGCACGC | (CCT)6 | 263 | 60 | ORF | 2 | 0.37 | JU981472 | hypothetical protein |
| R: TTGTCGAAGGAGTAGGAGGC |
Note: A = number of alleles detected; PIC = polymorphism information content; Ta = optimal annealing temperature.