Table 1.
Characteristics of 11 polymorphic and 26 monomorphic Pisum sativum EST-SSR markers.
| Locus | Primer sequences (5′–3′) | Repeat motif | Size (bp) | Ta (°C) | GenBank accession no. | Putative function [organism] | E-value |
| Psat61* | F: CCGGTTCGGTTTCCGGTTGAGG | (GGGTTC)4 | 81 | 60 | JR344273 | unknown | |
| R: ACGGACTCCAGCCAGCACCA | |||||||
| Psat900* | F: GCTGATCCCATTCCAACCACAGGC | (TTG)5 | 135 | 58 | JR344282 | chalcone reductase [Medicago sativa] | 7.44E-12 |
| R: ACAACCTTACCTTAAACCTTCTCAACC | |||||||
| Psat921* | F: TCAACTCTCAACAGGCGCTGC | (GCT)6 | 248 | 58 | JR344284 | unknown | |
| R: TGTCACAACGACCCTGCAAGC | |||||||
| Psat5404* | F: ACTTCACATTGCACTCTTTCTTCAC | (CT)5 | 103 | 56 | JR344267 | unknown | |
| R: TGAATCTCCCATATCTCAACTCAAGTG | |||||||
| Psat5545* | F: TCCCATGGAACAAGCTCATCATCC | (TCA)7 | 123 | 58 | JR344268 | predicted protein [Medicago truncatula] | 3.98E-05 |
| R: TGGGTTCAGTGAGGAACAGGT | |||||||
| Psat5571* | F: AGGAGCGGCTGAAGAAAGAGT | (AG)6 | 135 | 58 | JR344269 | predicted protein [Glycine max] | 5.32E-26 |
| R: CACCGCTGTAGAGGGCGTGA | |||||||
| Psat7112* | F: TGATGATGTGCTGATTATTGTTCTGGT | (TTA)5 | 170 | 58 | JR344274 | unknown | |
| R: ACAGTCACAGAAAGTGTCTACAGCA | |||||||
| Psat7598* | F: ACTACAGGAGTTGAATTTGCGGA | (GAT)6 | 209 | 54 | JR344275 | basic helix-loop-helix protein bhlh5 [Lotus japonicus] | 3.43E-04 |
| R: CAACATCAACAAGAACAAGAACACG | |||||||
| Psat7818* | F: TTGAGGTTGTTGTTGTTGTTGCTGT | (GTT)5 | 80 | 58 | JR344277 | predicted protein [Ricinus communis] | 1.82E-05 |
| R: AAACAAAGGAAGTTTGGGCAGC | |||||||
| Psat9662* | F: AGTGAAGCGAGTGGAAGATACGA | (GAAATC)5 | 171 | 58 | JR344287 | fiber protein fb11 [Camellia sinensis] | 5.59E-15 |
| R: GGCCAAAGCCGGCGATGAGA | |||||||
| Psat10014* | F: ATATCGCCACGACGCAAAGC | (GT)6 | 124 | 58 | JR344254 | unknown | |
| R: TCTTACATGACAAAGCCAACACAAG | |||||||
| Psat368 | F: ACATTCCTCCGGCGTAGCTGA | (AATCGG)4 | 81 | 58 | JR344261 | DNA repair protein Rad23-1 [Ricinus communis] | 1.28E-27 |
| R: ACAGTGAGCTTCATGACTACTCGGC | |||||||
| Psat373 | F: CCTGGTGATGCTCCTCAGGCA | (GAA)5 | 155 | 58 | JR344262 | transcription elongation factor family protein [Arabidopsis thaliana] | 3.31E-39 |
| R: TCAGCTGTAATCTCAAGCTCAGCCA | |||||||
| Psat589 | F: TGTGTAGCATCATCAGCGGAGC | (GA)5 | 162 | 58 | JR344271 | chitinase [Cicer arietinum] | 1.54E-96 |
| R: CCCGCAACTAAACCTTGCTGGC | |||||||
| Psat1176 | F: GCCTATTTGTACTATTCCACCACCTG | (TA)5 | 179 | 56 | JR344256 | unknown | |
| R: ACGGATGAATAAGTGACATTACAGTGA | |||||||
| Psat1764 | F: TCAGGGTCGGTGAGGCTTCGT | (GA)6 | 152 | 58 | JR344257 | predicted protein [Medicago truncatula] | 6.40E-11 |
| R: TCAGTGAAGAACATGGCACCAA | |||||||
| Psat2045 | F: GAAGCGGCGACGATGGCGTA | (TTG)5 | 224 | 58 | JR344258 | mannose-P-dolichol utilization defect 1 protein [Arabidopsis thaliana] | 5.18E-21 |
| R: AGTTCAGTTTGAGTAAACATTGACGG | |||||||
| Psat2885 | F: AGACGGAGGAGACGTGGAGGA | (GA)5 | 139 | 58 | JR344259 | unknown | |
| R: CACCACCACCAACGCCGTCA | |||||||
| Psat3352 | F: TGATTGGGATCGACTTCGACGG | (GA)5 | 100 | 58 | JR344260 | 40S ribosomal protein S5 [Cicer arietinum] | 2.99E-08 |
| R: AGAGCATTTGAAGTGTTTACGGCTGC | |||||||
| Psat4097 | F: GCCAAACATGCCAACAACAATCCT | (TTA)6 | 152 | 58 | JR344263 | unknown | |
| R: TCACTGAGCCACCGCCAACG | |||||||
| Psat4741 | F: CCACCACTTCAACCCTCTCAACGA | (GTG)5 | 123 | 56 | JR344264 | Phloem-specific protein [Pisum sativum] | 1.34E-24 |
| R: TCGACCGCTACCCAAACGCTG | |||||||
| Psat4773 | F: ACAGCTCCTGGCACAGCTCTT | (TA)5 | 178 | 58 | JR344265 | predicted protein [Glycine max] | 1.57E-25 |
| R: CCCAATTGCTTATGTCTGCTGCCT | |||||||
| Psat5398 | F: TCACCAATTCGCCCTCTCTCCA | (CT)5 | 131 | 58 | JR344266 | unknown | |
| R: CGCAAGGTTCCAGATTCTTCGAGGT | |||||||
| Psat5852 | F: TGCCAACCAGGTAGAGTCTCA | (CTT)5 | 151 | 56 | JR344270 | predicted protein [Medicago truncatula] | 8.04E-30 |
| R: AGTCGAATCTTGTTCCTTCTTCTTTGA | |||||||
| Psat6026 | F: TGTGCTTCTTGTGGCTGGTGA | (GA)5 | 134 | 58 | JR344272 | predicted protein [Glycine max] | 1.40E-34 |
| R: GTCCCTCGCGACGACACCAA | |||||||
| Psat7675 | F: TCACGTCGCTTCGTTTCATCCC | (TGA)6 | 183 | 56 | JR344276 | small heat shock protein 1 [Prunus salicina] | 2.94E-08 |
| R: ACCACCCATCACACCAAACCCA | |||||||
| Psat7820 | F: CCGGAGCGGAGGCGAAGAGA | (TTC)5 | 159 | 56 | JR344278 | predicted protein [Medicago truncatula] | 6.37E-35 |
| R: GGGACGCAGTAATCAACCAGA | |||||||
| Psat7825 | F: CCAGACACAGATCCTCAACAACTCCG | (CT)5 | 94 | 60 | JR344279 | intracellular chloride channel [Medicago truncatula] | 5.76E-28 |
| R: GCGGCGCACTTTCGTAGCAG | |||||||
| Psat8001 | F: TCTCCTCACAACTCAACTGTTACC | (TTC)7 | 141 | 58 | JR344280 | unknown | |
| R: TGGTGGTGAGACCGAGTGAGA | |||||||
| Psat8487 | F: TGTTTCCAGAAGGTTATGGCCC | (GA)5 | 155 | 54 | JR344281 | HXXXD-type acyl-transferase-like protein [Glycine max] | 8.16E-22 |
| R: AGATTCTTCGTTAGCCTTTGCTTTGA | |||||||
| Psat9191 | F: TGCAAACTTCAATGAGAGATCTGAAAG | (TA)5 | 158 | 56 | JR344283 | predicted protein [Medicago truncatula] | 2.03E-04 |
| R: TGCATTGGAGATGCCAAATCTGACT | |||||||
| Psat9319 | F: GCAGCACCACCACTCGCAGG | (CCA)5 | 105 | 56 | JR344285 | unknown | |
| R: AGCTGAGGTGATTGCTTCTGGT | |||||||
| Psat9501 | F: GCTTGCCTTTTGATTTTCCACGTCA | (CTT)5 | 196 | 56 | JR344286 | proteinase inhibitor I4, serpin [Medicago truncatula] | 1.77E-70 |
| R: TCATCGTGCGGTTGCACTTGT | |||||||
| Psat9677 | F: TGCAACAACTACGGATCACCAGC | (TTGTA)4 | 189 | 58 | JR344288 | unknown | |
| R: GCTGAACCAGATACACAAAGTTGAGC | |||||||
| Psat9736 | F: GGTCCTCCTCCAGGTTATGACCCTC | (GAAA)4 | 108 | 56 | JR344289 | ensangp00000004563 related [Medicago truncatula] | 6.40E-19 |
| R: AGTTTCCTTTACCTGAAGTCGTTTCT | |||||||
| Psat9907 | F: GACGGAACCGCCGTCCAACA | (GAC)5 | 162 | 58 | JR344290 | predicted protein [Glycine max] | 3.99E-05 |
| R: ACCACCTTGAGCGGCGTCAT | |||||||
| Psat10084 | F: TGCGGAGAAAGCGCTGCTGG | (AAG)5 | 110 | 56 | JR344255 | unknown | |
| R: ACGCAACCTTCTTCTTCTTCTTTCT |
Note: Ta = annealing temperature.
*
Polymorphic EST-SSR markers.