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. Author manuscript; available in PMC: 2015 Jun 9.
Published in final edited form as: Dev Cell. 2014 May 22;29(5):511–520. doi: 10.1016/j.devcel.2014.04.017

Figure 5. RARα, but not RARβ or RARγ, is required for transdifferentiation.

Figure 5

A-F, Immunofluorescence detection of SOX9 and FOXL2 proteins in adult testes. Conditional deletion of Dmrt1 with one copy of Rara using Sf1-Cre caused strong transdifferentiation (A), whereas deletion of Dmrt1 and both alleles of Rara with Sf1-Cre strongly suppressed transdifferentiation (B), indicating that Rara is required. By contrast, deletion of Dmrt1 alone or with Rarb using Sf1-Cre (C,D) or deletion of Dmrt1 with one (C) or both (D) alleles of Rarg using Dhh-Cre caused transdifferentiation, indicating that Rarb and Rarg are not required for transdifferentiation of Dmrt1 mutant Sertoli cells. Scale bars, 80 µm.